電子顕微鏡用包埋樹脂

電子顕微鏡、光学顕微鏡用包埋剤

　　　　　　　　　　　エポキシ樹脂　メタクリル酸樹脂　ポリエステル樹脂　光顕用樹脂　　　　　　　　　　　　

エポキシ樹脂

#02-1001 Oken　Epok　８１２　セット　\13,000.-

セット内容：

エポック８１２　５００ｇｘ２
ＤＤＳＡ　５００ｇｘ１
ＭＮＡ　５００ｇｘ１
ＤＭＰ－３０　５０ｇｘ１

Epon　８１２（Shell　Chemical　USA）の製造中止に伴い国産化されたEpoxy　Resinです。　Luftの処方に準じたEpon８１２の処方で使用することが出来ます。

※Epok８１２の性状　エポキシ当量　１４３（WPE）、粘度　１６０（CPS。25℃）、比重　１．２４　（25℃）

Epok８１２の混合液の組成		A:B＝１：１の場合
混合液A（硬化物が軟らかい）	混合液B（硬化物が硬い）	Epok８１２　４６ｍｌ DDSA　３０ｍｌ MNA　２４ｍｌ DMP-30　１．５ｍｌ
Epok８１２　６２ｍｌ DDSA　１００ｍｌ DMP-30　１．５％	Epok８１２　１００ｍｌ MNA　８９ｍｌ DMP-30　１．５％
A液：B液＝６：４～４：６

エポック８１２の特長

Luftの方法で包埋が出来ます。
粘度が低いので浸透性が良い。
広範囲に硬さを調節出来る。

混合液は60℃にて約12時間で硬化する。

#02-1002　Oken　Epok ８１２	200g	\2,300.-	（主剤）
#02-1003　Oken　Epok ８１２	500g	\4,500.-	（主剤）
#02-1004　Oken DDSA	200g	\1,500.-	（硬化剤）無水ドデセニールコハク酸）
#02-1005　Oken DDSA	500g	\3,000.-	（硬化剤）無水ドデセニールコハク酸）
#02-1006　Oken　MNA	200g	\1,500.-	（硬化剤）無水メチルナディック酸）
#02-1007　Oken　MNA	500g	\3,000.-	（硬化剤）無水メチルナディック酸）
#02-1008　Oken　DMP-30	25g	\500.-	（重合促進剤）
#02-1009　Oken　DMP-30	50g	\1,000.-	（重合促進剤）

アラルダイト樹脂（Araldite　Resin）

信頼性が高く多用途のアラルダイトCY－２１２は、薄切が容易、且つ、電子線に対して高い安定性を持っています。

#02-1010 Oken　Araldite　M(CY-212)　セット　\11,000.-

セット内容：Araldite　M　（CY-212）　５００ｇ　ｘ　１
　　　　　　　Hardener　（９６４B硬化剤）　５００ｇ　ｘ　１
　　　　　　　Acceleator　（９６４C加速剤）　５０ｇ　ｘ　１
　　　　　　　Dibuthyl　Phtalate　１００ｇ　ｘ　１

#02-1011 Oken　アラルダイト　M	５００ｇ	\5,000	CY-212
#02-1012 Oken　アラルダイト　M	１Kgrms		中止品

メタクリル酸樹脂（Methacrylate　Resin）

粘性が低く、試料の急速浸透を可能にし、簡単に切片化できるブロックを作ります。　ブロックの硬度はメタクリル化合物の配合率によって決まります。

#02-1013 Oken　メタクリル酸メチル　（ＭＭＡ）	５００ｇ	\4,500
#02-1014 Oken　メタクリル酸ブチル　（ＢＭＡ）	５００ｇ	\4,500
#02-1015 Oken　スチレンモノマー　（Ｓｔｙｒｅｎｅ　Ｍｏｎｏｍｅｒ）	５００ｇ	\3,500
#02-1016 Oken　過酸化ベンゾイルペースト（BPOペースト）５０％	25g	\500

水溶性メタクリレート樹脂

本水溶性キットを使用すればエタノール、アセトンの脱水剤が不必要です。

#02-1017 Oken　Metholate　G（GMA）セット　\6,500.-　（２－Hydroxyethyl　Methacrylate）

セット内容　Metholate　G　５００ｇ
　　　　　　　２－２－Azobis-iso-butyronitrile　１０ｇ
　　　　　　　Ammonium　Persulfate　１０ｇ
　　　　　　　BPO　１０ｇ

※別売品　#02-1018 Metholate　G　５００ｇ　\5,000.-

#02-1019 Oken　Metholate　HP（HPMA）セット　\6,500.-　（２－Hydroxypropyl　Methacrylate）

ヒドロキシプロピル・メタクリレートは水溶性モノマーで、試料作成中に使用するエタノール、アセトン等の脱水剤によって生じる種々の問題を防ぐために使用されます。

セット内容　Metholate　HP　５００ｇ
　　　　　　　２－２－Azobis-iso-butyronitrile　１０ｇ
　　　　　　　BPO　１０ｇ

※別売品　#02-1020 Metholate　HP　５００ｇ　\5,000.-

ポリエステル樹脂（Polyester　Resin）

リゴラック（Rigolac）は包埋の際収縮が少なく、発泡がなく、均一な反応をおこす点で優れております。　触媒は反応の緩やかなBPOペースト（過酸化ベンゾイルをジブチルフタレートで溶解した物）を使用します。　以前販売しておりましたパーメックN,プロモーターEは取り扱いによっては爆発の危険性がありますので取り扱いを中止しております。

#02-1021 Oken　リゴラック　７０F	500g	\3,000	Rigolac　７０F
#02-1022 Oken　リゴラック　２００４	500g	\3,000	Rigolac　２００４
#02-1023 Oken　スチレンモノマー	500g	\3,500	Styrene　Monomer
#02-1024 Oken　過酸化ベンゾイルペースト	25g	\500	BPOペースト（加熱長時間硬化用触媒）

TAAB社包埋用樹脂

Epon 812 is the triglycidyl ether of glycerol and was developed in the USA as an alternative to Araldite CY212. It is used in a combination with DDSA and methyl nadic anhydride (MNA) and is rather less viscous than Araldite mixtures. A suitable mixture for medium hardness blocks is:

Epon 812 48.0 parts DDSA 19.0 parts MNA 33.0 parts DMP-30 2.0 parts

Harder blocks may be obtained by replacing part of the DDSA by MNA and softer blocks by replacing part of the MNA with DDSA. Some experimentation is worthwhile to achieve the perfect formulation for the tissue most commonly sectioned.

#02-1025 TAAB社　Epon 812 Resin Kit　\38,000.-

セット内容：　Epon　８１２　１００ｇｘ５
　　　　　　　　DDSA　EM　　１００ｇｘ３
　　　　　　　　MNA　１００ｇｘ３
　　　　　　　　DMP-30　２５ｇｘ1

#02-1026 TAAB Epon８１２ Resin	１００ｇ	\3,900.-	#02-1032 TAAB MNA	１００ｇ	\2,600.-
#02-1027 TAAB Epon ８１２Resin	２５０ｇ	\7,700.-	#02-1033 TAAB MNA	２５０ｇ	\5,500.-
#02-1028 TAAB Epon ８１２Resin	５００ｇ	\15,000.-	#02-1034 TAAB MNA	５００ｇ	\8,500.-
#02-1029 TAAB DDSA　EM　Grade	１００ｇ	\3,200.-	#02-1035 TAAB DMP-30	２５ｇ	\1,500.-
#02-1030 TAAB DDSA　EM　Grade	２５０ｇ	\6,400.-	#02-1036 TAAB DMP-30	５０ｇ	\2,000.-
#02-1031 TAAB DDSA　EM　Grade	５００ｇ	\12,000.-	#02-1037 TAAB DMP-30	１００ｇ	\3,000.-

パラプラストプラス PARAPLAST PLUS 502004 (Tissue　Embedding　Medium　ｂｙ McCormick Scientific )

A compound of purified paraffin and plastic polymers of regulated molecular weights. Contains Dimethyl sulphoxide (DMSO) for rapid tissue infiltration.

Paraplast Plus tissue embedding media is a mixture of highly purified paraffin containing a plastic polymer. Paraplast Plus contains a small percentage(0.8%) of dimethyl sulphoxide(DMSO). The plastic polymer combined with paraffin yields a media whose physical properties enhance quality and ease of sectioning. DMSO in paraplast Plus further enhances these properties.

Pellet-form provides easier handling and faster melting characteristics.
Contains DMSO for rapid tissue infiltration-cuts cleanly and smoothly without cooling.
Assures excellent ribbon continuity with serial sections to 2 microns-no crumbling or cracking.
Free of foreign contaminants-double filtered through our completely automated manufacturing process.

※製造メーカー、以前のKendall社からMcCormick社に変わっておりますが、内容等に付きましては全く同じ商品です。

価格　#02-1038 パラプラストプラス　1kg.(2.2lbs.)/Bag. \3,500.- #02-1039 パラプラストプラス　1kg. 8個入　1箱　\25,000.-

#02-1040 Poly/Bed　８１２　Resin Kit　（Polyscience社）　\33,000.-

Polysciences has been the leader in supplying epoxy embedding material to microscopists for many years. Poly/Bed line of products are the reliable, long-established means by which you can be assured of secure specimen embedment. Use Poly/Bed for your scientific studies requiring beam stability, low shrinkage, and good contrast. Poly/Bed Epoxy formulations are of lower viscosity than Araldite formulations, and hence provide better penetration into tissue samples.
Contents of kit: 1x500gm #02-1041Poly/Bed 812\11,000.-, 1x450g #02-1042Dodecenyl succinic anhydride(DDSA)\10,000.-, 1x450g #02-1043Nadic Methyl Anhydride(MNA)\10,000.-, 1x100g #02-1044DMP-30\3,500.-, complete instrucdtions included.

#02-1045 スパーキット（Polyscience社） Spurr Low-Viscosity Embedding Media　\35,000.-

本キットは、2006年に発表された　Dr. E. Ann Ellis　の文献に従い改良が行われたため、キット内に含まれるVCD　２５０ｇが、ERL4221　２５０ｇへ変更になっていますが、性能は以前のものと変わらず使用が可能です。

NSA 450gx1 DER-736 250gx1 ERL-4221 250gx1 DMAE 100gx1

For Biological, Material and Mineralogical Specimen.

Background and Advantages: The introduction of Spurr resin provided the microscopist with a resin of exceptional penetration qualities. Unlike the other epoxies, Spurr resin with a viscosity of 60 cps will readily penetrate membrane walls, hard tissue, rocks and other minerals. It has also been used successfully in preparations of specimens with a high lipid content, tissues with hard, lignified walls, and highly vacuolated parenchymatous tissue.

The Spurr resin is based on the four following components. ERL 4221, which because of its compact structure yields highly cross-linked polymers with good high temperature resistance.

The flexibilizer is the epoxy resin, D.E.R.736,(diglycidyl ether of polypropylene glycol). This has a WPE value of 175-205. The amount of D.E.R. 736 may be regulated to provide embedments with a variety of hardness characteristics.

NSA, Nonenyl succinic anhydride is the hardener specially purified for EM usage. To avoid hydorlysis of epoxide or anhydide bond, we recommend minimum exposure of the above to atmospheric moisture. The accelerator is demethylaminoethanol(DMAE). This agent gives longer pot life the resin with a lighter color. Curing times can be shortened by increasing the accelerator ratios.

Lowicryl Embedding Media

Highly crosslinked acrylate based embedding media for light and electron microscopy
Low viscosity for better infiltration, even at low temperatures
Provides preservation of protein and membrane sturucture
Polymerized by UV at both low and room temperatures; may also be chemically polymerized at 60ºC

#02-1046 Lowicryl　樹脂　K4M　（水溶性）　\95,000.-

Hydrophilic embedding media combines low viscosity and water-miscible properties of methacrylates without melting phenomenon typically occurring in the electron microscope. Infiltrates and polymerizes as low as -35ºC by UV. Also useful for immuno-staining technique.

K4Mを使うとより良い構造の保存、抗原性の保存の改善、非常に低いバックグランドラベルが出来ます。

Kit includes: (a)750ml Monomer (b)132ml Crosslinker (c)4.7g Initiator

メタアクリル酸エステルに基づいた包埋剤、低温では低粘性を示す。　-35℃までイオン化（ハイドロフィリック）、長い波長（３６０ｍｍ）の紫外線の光によって光重合される。　光重合の場合は温度に影響を受けないので、ブロックは浸透時と同じ温度で重合される。　又、室温重合、オーブン内（60℃）で化学的にも重合される。　特殊な抗血清や、レクチンを使った断片の免疫表示に特に有効である。

#02-1047 Lowicryl　樹脂　HM20　（疎水性）　\95,000.-

Hydrophobic embedding media polymerizes as low as -70ºC by UV.

Kit includes: (a)750ml Monomer (b)132ml Crosslinker (c)4.7g Initiator

低温非イオン化レジンでクロス結合したメタクリル酸エステル/アクリル方式に基づいている。　HM-20は-70℃まで使用できる。　HM-20もK4Mと同様、長い波長（３６０ｍｍ）の紫外線の光によって光重合が出来る。　Z－コントラストによって、完全に汚れのない薄い断片の高いコントラスト画像が作れます。

#02-1048 Lowicryl　HM23　（疎水性）　中止品

A new alternative nonpolar embedding media, similar to HM20, yet useful for inliltration and polymerization at temperatures as low as -80ºC.

Kit includes: (a)750ml Monomer (b)132ml Crosslinker (c)4.7g Initiator

#02-1049 Lowicryl　樹脂　K11M　（親水性）　中止品

A new alternative polar embedding media, similar to K4M, yedt useful for infiltration and polymerization at temperatures as low as -60ºC.

Kit includes: (a) 750ml Monomers (b) 132ml Crosslinker (c) 4.7g Initiator

注①ＨＭ２３及びＫ１１ＭはＫ４ＭとＨＭ２０に似た性質を持つ、しかし20℃の低温でしようすることが出来、又、20℃以下でイオン化出きる。
　②Ｋ４Ｍで重合包埋の際はＤＳＫ紫外線重合装置TUV-100、TUV-200型をご利用いただきますと非常に良い結果が得られます。

光学顕微鏡包埋樹脂及び器具

#02-1050 光顕用樹脂　JB-4キット　\55,000.-

キット内容：　Ａ液　800mlx1　　Ｂ液　30mlx1　触媒　12gx1　

本キットは光顕用で可塑性があり、以下の理由でパラフィンに替わるものとして開発されました。

試料の完全な脱水が可能である。
常温またはそれ以下の浸透が可能である。
パラフィン使用の場合より薄い切片が得られる。
染色に際してはあらゆる水溶性組織染色剤が使用でき、より詳細な構造が観察できる。

Tissue embedded in JB-4 Embedding Medium offers superior ultrastructural preservation and semi-thin sections for high resolution light microscopy. This water solubleplastic media can replace most embedding procedures in which pafaffin or epoxy resins are used, and offers the advantage of less distortion and tissue shrinkage. Difficult specimesn, such as undecalcified bone and delicate embryonic tissue can be easily processed in JB-4 plastic.

Sections of spleen showing comparison of specimen embedded in JB-4 (left), and paraffin (right). hematoxylin/Eosin stain, oil lens x 1000.


Photograph courtesy of Nate Brinn, Dept. of Pathology, Duke University Medical School, North Carolina.

Candida albicans, specimen from a kidney taken at autopsy embedded in JB-4. PAS/lightgreen stain, x 400 (Acknowledgment: Burns, WA, Bretschneider, A: This is in: Plastic Embedding of Tissue for Light Microscopy. Chicago: Amerifcan Society of Clinical Pathologists, Used by Permission)
Both aqueous and non-aqueous fixatives can be utilized with JB-4 embedding medium including OsO4 and neutral buffered fixatives. Unlike paraffin, removal of JB-4 plastic prior to staining is unnecessary and brilliantly clear saining has been achieved using water soluble dyes. JB-4 does not interact with any tissue group pf importance for staining during polymerization, contrast to epoxy resins which may alter the staining character of tissue and are less responsive to histological stains.

Extremely fast processing times are possible with JB-4 embedding medium as only a few simple processing steps are required. Since JB-4 is water soluble, there is no need to completely dehydrate the tissue before infiltrating. A graded series of ethyl alcohols through 95% is commonly used to dehydrate tissue. For the retention of lipids and enzymes, precessing tissue at 4º C is recommended.

On a routine basis, 1-2µ thick sections are easily cut with a glass knife. Recently some investigators have even utilized conventional rotary microtomes for semi-thin sections of JB-4 embedded material. Under the same conditions, paraffin sections must be cut 5-6µ thick with much cellular overlap and the loss of clear cellular resolution. Traditional hard embedding plastics, such as epoxies or polyesters are more difficult to sections at the 1-2µ level and often result in wrinkling and compression.

JB-4 has been used sucessfully in enzyme histochemistry, immunohistochemistry techniques, and high resolution autoradiography sections, JB-4 plastic enhances the ability to identify labelled cells.

The increased unclear differentiation obtained with JB-4 plastic is a great aid to diagnosis, especially with liver and kidney tissue where fine cytogogical detail and rapid processing is necessary. Such morphological evaluation from needle biopsies is not possible with paraffin or other plastic materials.

血管造影剤（Microfil　Silicon　Rubber　Injection　Compound）　Flow　Tek　社製

血管注入剤　Microfil　の素材はシリコンラバーで、樹脂状の血管注入剤とは異なり、決して硬化はせず、注入した臓器からも通常のパラフィン包埋組織標本を作製する事ができます。　Microfil　の最も優れている点は、微細な毛細血管レベルまで良好な血管網が描出される点で、通常のバリウムゼラチン法とは比較にならないほどの美しさです。

極めて微小なレベルまで良好な充溢感が得られ、従来のバリウムゼラチン法をはるかに凌駕しています。
簡単にブレンドが得られます。
透徹法による立体観察の外、通常の組織検査にも用いられ、また軟X線撮影も可能です。


MICROFIL MV-130 (Red) injection of rat Kidney. Courtesy robert A. Acland, University of Louisville	Vasa vasonrum of the human coronary artery filled with MICROFIL Compound. Courtesy of A.C.Barger,R.Beeuwkes lll,L.L. Laney and K.J.Silverman, Harvard Medical School

MICROFIL compounds will fill and opacify microvascular and other spaces of non-surviving animals and post-mortem tissue under physiological injection pressure. The continuous, closed vascular system lends itself to flow through or perfusion techniques. Following injection, MICROFIL compounds cure to form a three-dimensional cast of the vasculature.

MICROFIL MV-series compounds are available in five radiopaque colors, as well as clear. MV-series compounds require either an alcohol-methyl salicylate or glycerin clearing sequence, whereby the refractive index of the clearing solution is the same as the refractive index of the tissue. This allows for microscopic examination of a selected vasculare bed.

MICROFIL CP-101 compound is intended for use in cast-corrosion techniques, and is designed for filling large blood vessels(greater than 100microns). Although the CP-101 compound will fill capillaries, these vessels fragment when the supporting tissue is removed through exposure to a potassium hydroxide solution. When cured, MICROFIL CP-101 is milky white in color, Casts made using CP-101 will maintain their dimensional accuracy indefinitely.

Advantages offered with MICROFILL compounds over previously available rubber injection materials include:

Complete filling with minimal shrinkage, to enhance vessel continuity and to produce in cleared preparations a vivid, optically cleared specimen that allows a precise study of the microcirculation.
Color diversity, to provide delineation within the circulatory tree for microscopic examination and photographic illsustration.

Areas of investigation

In physiology, MICROFIL visualization provides a means for establishing the precise vascular architecuture of specific organs allowing comparison between normal and abnormal structure.

In surgery, visualization of the microcirculation and microanatomy is leading to improved surgical techniques in the repair of nerves, tendons, and blood vessels.

In gastrointestinal research, MICROFIL compounds characterize and describe changes in vascular patterns associated with several pathological conditions.

Injected specimens, when preserved in methyl salicylate or glycerin, also serve as a definitive teaching adjunct.

	MV-112	MV-117	MV-120	MV-122	MV-130	MV-132	CP-101
Color	White	Orange	Blue	Yellow	Red	Clear	Milky White
Specifi gravity	1.04	1.02	1.00	1.04	1.02	1.00	0.98
Viscosity, centipoise	35	25	25	25	30	20	25
Gel time, minutes	90	90	90	90	90	90	45
Useful shelf life, months	4	4	4	4	4	4	6

価格：#02-9999 Bキット　1ポンド入り（１色）\59,000.-と#02-9999 Cキット　2ポンド入（1色又は２色）\92,000.-があります。

ご注文の際は色の種類、Bキット或いはCキットの指定を明示ください。

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